The influence of lipid membranes on fluorescent probes' optical properties

Organic fluorophores embedded in lipid bilayers can nowadays be described by a multiscale computational approach. Combining different length and time scales, full characterization of the probe localization optical properties led to novel insight into effect environments. Following an introduction on advancements, three relevant probes are reviewed that delineate how approach lead probes' (non) linear properties. Attention is paid quality theoretical description techniques. Computation assess priori guide analysis interpretation experimental data studies. The used gain information phase condition surrounding biological environment. showed canonical view some should revisited adapted. • Laurdan, DiI azobenzene based model bilayers. Their location orientations simulated using molecular dynamics calculations. Multiscale modelling accesses (non)linear fluorescence Probes' depend both environment conformation. Computations provide evidence adapt views experiments.